human bladder carcinoma cell line j82  (ATCC)

Journal: The FASEB Journal

Article Title: Intravesical Delivery of P21 mRNA –Loaded Lipid Nanoparticles as a Tumor Suppressor Replacement Therapy for Bladder Cancer

doi: 10.1096/fj.202600049R

Figure Lengend Snippet: Rationale for selecting CDKN1A /p21 as a therapeutic target in bladder cancer and validation of p21 mRNA expression. (A) Pan‐cancer analysis of CDKN1A alteration frequencies based on TCGA datasets. The analysis was performed using the TIMER3 web server. (B) CDKN1A mRNA expression in bladder cancer and normal bladder tissues, shown as log2(TPM + 1), where TPM denotes transcripts per million. Data were analyzed using the TIMER3 web server. (C) Representative p21 immunohistochemical staining images from a bladder cancer tissue microarray stratified by pathological stage (Tis, T1, T2, T3, and T4). Scale bars, 200 μm. (D) Quantification of p21 H‐scores in Tis, T1, T2, T3, and T4 lesions. Compared with Tis lesions, T1, T2, T3, and T4 tumors showed significantly lower p21 H‐scores. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01 (one‐way ANOVA). (E) Western blot analysis of basal and p21 mRNA‐induced p21 protein expression in T24 and J82 bladder cancer cells. Basal p21 protein levels were very low in both cell lines, whereas transfection of p21 mRNA led to robust p21 expression. For p21 blots, both short‐exposure (se) and long‐exposure (le) images are shown. β‐Actin serves as a loading control. (F) Immunofluorescence analysis of EGFP‐HA and p21‐HA expression in T24 and J82 cells using an anti‐HA antibody. Nuclei were stained with DAPI. The p21‐HA signal was predominantly localized to the nucleus, whereas EGFP showed diffuse cytoplasmic distribution. Scale bars, 20 μm.

Article Snippet: The human bladder carcinoma cell line J82 and the human embryonic kidney cell line HEK293T were purchased from the American Type Culture Collection (ATCC).

Techniques: Biomarker Discovery, Expressing, Immunohistochemical staining, Staining, Microarray, Western Blot, Transfection, Control, Immunofluorescence

Journal: Cancers

Article Title: A Novel Cyclic Pentadepsipeptide, N -Methylsansalvamide, Suppresses Angiogenic Responses and Exhibits Antitumor Efficacy against Bladder Cancer

doi: 10.3390/cancers13020191

Figure Lengend Snippet: MSSV-mediated inhibition of proliferation of the human bladder cancer cell lines, 5637 and T24, was owing to G1-phase cell cycle arrest. ( A ) Both cell lines were treated with or without MSSV at the indicated concentrations for 24 h, followed by MTT assays for cell viability. ( B ) Cells were treated with indicated concentrations of MSSV for 24 h, and cell counting was performed via trypan blue staining. ( C ) Both cell lines were treated with MSSV for 24 h and analyzed for cell cycle distribution using fluorescence-activated cell sorting (FACS) histograms. The percentage of cells in each cell cycle phase is presented. ( D ) Cells were exposed to MSSV at the indicated concentrations for 24 h. The expression levels of cyclin D1, cyclin E, CDK2, CDK4, p21WAF1, p27KIP1, p53, and GAPDH were analyzed via immunoblotting. Bar graphs show the relative fold changes in proteins at different MSSV concentrations in comparison with the control. ( E ) The cell lysates were immunoprecipitated with antibodies recognizing CDK2 and CDK4, followed by immunoblotting with specific antibodies against p21WAF1, p27KIP1, CDK2, and CDK4. Graphs show the relative amount of immunoprecipitated proteins as fold changes in comparison with the control. For the bar graphs, values were presented as the mean ± SD of three independent experiments; * p < 0.05, compared with the control group. Uncropped Western Blot Images in .

Article Snippet: The human bladder carcinoma cell lines, 5637 and T24, were purchased from the American Type Culture Collection (Manassas, VA, USA) and maintained in Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C in a 5% CO 2 humidified incubator.

Techniques: Inhibition, Cell Counting, Staining, Fluorescence, FACS, Expressing, Western Blot, Comparison, Control, Immunoprecipitation

Journal: International Journal of Nanomedicine

Article Title: Inhibitory effect of Au@Pt-NSs on proliferation, migration, and invasion of EJ bladder carcinoma cells: involvement of cell cycle regulators, signaling pathways, and transcription factor-mediated MMP-9 expression

doi: 10.2147/IJN.S158463

Figure Lengend Snippet: Inhibition of the proliferation of EJ bladder cancer cells by heterometallic Au@Pt-NSs. Notes: Both EJ cells and HUCs were incubated for 24 h with different concentrations of Au@Pt-NSs (0, 0.1, 0.3, and 0.5 μM). Cell viability was measured using the MTT assay in EJ cells ( A ) and HUCs ( D ). Viable cells were measured using trypan blue staining for EJ cells ( B ) and HUCs ( E ). Morphological changes were observed after treatment with Au@Pt-NSs at different concentrations (0, 0.1, 0.3, and 0.5 μM) for EJ cells ( C ) and HUCs ( F ). For the bar graphs, values are presented as mean ± SD of three independent experiments; * P < 0.05, compared with the control group. Abbreviations: Au@Pt-NSs, gold@platinum nanoseeds; Con, negative control; HUCs, human urothelial cells; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.

Article Snippet: The EJ human bladder carcinoma cell line was purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained in Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37°C in a 5% CO 2 humidified incubator.

Techniques: Inhibition, Incubation, MTT Assay, Staining, Control, Negative Control